Buy Phosphate Buffered Saline (PBS) pH 7.2 | Overnight Shipping
Buy Phosphate Buffered Saline pH 7.2 Online. Phosphate Buffered Saline (PBS) pH 7.2 is a sterile-filtered, isotonic, and non-toxic buffer solution commonly used in biological research. This solution is particularly essential for applications such as cell culture, washing, and immunohistochemical testing. Importantly, PBS maintains a stable pH of 7.2–7.4, which is suitable for mammalian cell maintenance, tissue disaggregation, and monolayer dispersal. This stability notably helps prevent cell rupture due to osmotic pressure.
Common Uses and Applications
Cell Culture:
First and foremost, PBS is widely used in cell culture for washing, dilution, and maintenance of cells without toxicity. This property ensures that cell viability is preserved during experiments.
Molecular Biology
Additionally, PBS is ideal for molecular biology applications, particularly in immunohistochemistry and hematological procedures. Its pH stability enhances the reliability of test results.
Tissue Handling
Furthermore, PBS is employed in tissue handling for tissue disaggregation and dispersal. This is particularly effective because lower magnesium and calcium ion concentrations do not hinder trypsin activity, facilitating efficient tissue processing.
Diagnostic Diluents
Moreover, PBS serves as a dilution buffer in diagnostic testing for water, food, and dairy products. Its reliability makes it a preferred choice in various testing environments.
Key Formulation Characteristics (pH 7.2)
Appearance and Composition
In terms of appearance, PBS is a colorless, clear liquid. The typical composition includes:
– Sodium Chloride (\(NaCl\))
– Potassium Chloride (\(KCl\))
– Sodium Phosphate (\(Na_{2}HPO_{4}\))
– Potassium Phosphate (\(KH_{2}PO_{4}\))
Storage Conditions
For optimal performance, PBS is stable at room temperature. However, prepared media is often best stored at 2–8°C to maintain its effectiveness over time.
Typical Preparation Components (1L)
The typical preparation for 1 liter of PBS includes:
– 80.1 g \(NaCl\)
– 14.4 g \(Na_{2}HPO_{4}\)
– 2 g \(KCl\)
– 2.7 g \(KH_{2}PO_{4}\)
To prepare, adjust the solution to 1 liter and modify the pH to 7.2 using \(HCl\).
Availability of PBS
Finally, both ready-to-use liquid and concentrated 10X formulations of PBS are available from reputable suppliers, including Sigma-Aldrich and HI Media Laboratories. This accessibility ensures researchers can easily obtain the buffer solution necessary for their experiments.
